Spatial Gene Expression of Mouse kidney Formalin-fixed paraffin embedded

Stereo-seq N FFPE V1.1, software version: SAW 8.2 & StereoMap 4.2
Mouse kidney Demo Data
Chip SN: Y40568D8
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
  • Species: Mouse (Strain: C57BL/6; Sex: Male; Age: 6-8 week)

  • Tissue: Kidney

  • Embedding method: Formalin-fixed paraffin embedded

  • DV200>70%

Kit information
  • Product: Go Spatial Stereo-seq OMNI FFPE

  • Kit version: Stereo-seq N FFPE V1.1

  • Chip size: 1 cm × 1 cm

Experiment information
  • Section thickness: 5 μm

  • Staining type: ssDNA staining

  • Permeabilization time: 30 min

Sequencing
  • Total reads: 1,724,553,246

  • Sequencer: DNBSEQ-G400RS

Analysis result
STOmics Rat embryo Intestine Demo Data Clustering map STOmics Rat embryo Intestine Demo Data Clustering map STOmics Rat embryo Intestine Demo Data Clustering map
Clustering map
  • Analyze Software Version: SAW 8.2, StereoMap 4.2

  • Bin200 (100 µm) Median MID: 44,237

  • Bin200 (100 µm) Median Gene Type: 9,430

  • Bin20 (10 µm) Median MID: 419

  • Bin20 (10 µm) Median Gene Type: 305

  • Cell Bin Gene Type: 240

  • Cell Bin MID: 317

  • Sequencing Saturation: 58.9%

  • Fraction MID in Spots Under Tissue: 89.69%

Input files Output and supplemental files
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Output and supplemental files Format Size md5sum
Download Selected
Y40568D8_output_command_download TXT
Y40568D8.adjusted.cellbin.gef GEF 1.40GB 03bdb7939efe61ccd78d7117674f82d0
Y40568D8.cellbin.gef GEF 975.98MB a6ff8593d39995fab7d97a4012d6bab6
Y40568D8.raw.gef GEF 1.34GB e30e952edc5653f06871348a50ed1f82
Y40568D8.report.html HTML 77.81MB e1465e386f363369da25b4baf7139253
Y40568D8_ssDNA_regist.tif TIFF 198.50MB 1c159300d7c81bccfd30334a9ffd70a4
Y40568D8.tissue.gef GEF 6.79GB bddbd21c0ebe8142ec1886e0169c03ce
Y40568D8.visualization.tar.gz TAR 5.90GB 0bb777d7ba42837eb948a3569ffb8b52
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