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Spatial Gene and Protein Expression of Mouse Spleen Fresh Frozen Tissue

Stereo-CITE T FF V1.1, software version: SAW 8.1 & StereoMap 4.1
Stereo-CITE Solution
Mouse spleen Demo Data
Chip SN: C04776D6
Sample information
Kit information
Experiment information
Sequencing
Analysis result
Input file & Output and supplemental files
Data Details
Sample information
Species : 
Mouse (Strain: C57BL/6; Sex: male; Age: 6-8 week)
Tissue : 
Spleen
Embedding method : 
Fixed Frozen
RIN value : 
6.5
Kit information
Product : 
Stereo-CITE FF
Kit version : 
Stereo-CITE T FF V1.1
Chip size : 
1 cm × 1 cm
Experiment information
Section thickness : 
10 μm
Staining type : 
DAPI staining
Permeabilization time : 
12 min
Sequencing
Total reads : 

2,727,841,217 (RNA)

1,546,520,159 (Protein)

Sequencer : 
DNBSEQ-T7RS    
Analysis result
Mouse spleen demo data Mouse spleen demo data Mouse spleen demo data
Clustering map

  • Analyze Software Version : SAW V8.1, StereoMap V4.1

  • Number of Proteins : 128

  • Bin200 Median MID : 92,454(RNA), 332,155(Protein)
  • Bin200 Median Gene Type : 11,526
  • Bin20 Median MID :  942(RNA), 3,299(Protein)
  • Bin20 Median Gene Type : 646

  • Cell Bin Gene Type : 354

  • Cell Bin MID : 480(RNA), 1,596(Protein)
  • Sequencing Saturation : 75%
  • Fraction MID in Spots Under Tissue : 77.35%
  • Manual tissue segmentation was performed.

Explore View report
Input files Output and supplemental files
Select All
 Output and supplemental files Format Size md5sum
Download Selected
C04776D6_output_command_download TXT
C04776D6_DAPI_regist.tif TIFF 147.22MB 4eed766e720430706812ba58f3c2d5d7
C04776D6.protein.adjusted.cellbin.gef GEF 413.98MB 3656ab33f4fe3950704e4a233702450d
C04776D6.protein.cellbin.gef GEF 386.69MB 6ab0f999756134f54f7a85aba7a6b4eb
C04776D6.protein.tissue.gem.gz GEM 1.44GB 830da04bf8d2bf7bad88a7b48268a32d
C04776D6.protein.tissue.gef GEF 7.86GB 71a137fd34f41959a5418f269f0377c8
C04776D6.protein.raw.gef GEF 5.26GB d414f9a53fae217faf68d98a0656b800
C04776D6.adjusted.cellbin.gef GEF 1.86MB 833d0e5aeda370d98a6ba253c867fb03
C04776D6.cellbin.gef GEF 1.28MB d940260e58f299719978467252e9c9dd
C04776D6.tissue.gem.gz GEM 794.43MB ce2a9ebe41075aa77e273e31c492aa2c
C04776D6.tissue.gef GEF 9.84GB 31062fa681ae41d2b771c4813ff5d43b
C04776D6.raw.gef GEF 2.10GB cd5320d753e2d1efaee489daf678e6a4
C04776D6.report.html HTML 70.88MB ce24c8f5d05c937f7ce25a1a323be8e9
C04776D6.visualization.tar.gz TAR 8.51GB ae315ffbb60c9b086b23854fb30d42e4
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