Square Bin-Protein

Analytical Report
A02677B5_DAPI_Demo_Realign
Alerts
Metrics
Value
Alert
Note

- Summary
  Gene Protein
- Square Bin
  Gene Protein
- Cell Bin
  Gene Protein
- Image
- Analysis

Tissue Square Bin Statistics

###### Bin Size

The size of Bin which is the unit of aggregated DNBs in a squared region (i.e. Bin 50 = 50 * 50 DNBs)

###### Mean Reads (per bin)

Mean number of sequenced reads divided by the number of bins under tissue coverage

###### Median Reads (per bin)

Median number of sequenced reads divided by the number of bins under tissue coverage (pick the middle value after sorting)

###### Mean MID (per bin)

Mean number of MIDs divided by the number of bins under tissue coverage

###### Median MID (per bin)

Median number of MIDs divided by the number of bins under tissue coverage
Bin Size
Mean Reads
(per bin)
Median Reads
(per bin)
Mean MID
(per bin)
Median MID
(per bin)
20
5,721
5,709
1,218
1,220
50
35,179
35,552
7,494
7,597
200
521,635
550,842
111,119
117,648

- Bin 20
- Bin 50

Distribution
Violin plots show the distribution of deduplicated MID count in each bin
Clustering
(left) Clustering spots (bin20) under tissue covered with the Leiden algorithm
(right) UMAP projections of spots (bin20) colored by automated clustering. Same color is assigned to spots that are within shorter distance and with similar gene expression profile
Tissue Plot with Spots (bin20)
UMAP Projection of Spots (bin20)
Distribution
Violin plots show the distribution of deduplicated MID count in each bin
Clustering
(left) Clustering spots (bin50) under tissue covered with the Leiden algorithm
(right) UMAP projections of spots (bin50) colored by automated clustering. Same color is assigned to spots that are within shorter distance and with similar gene expression profile
Tissue Plot with Spots (bin50)
UMAP Projection of Spots (bin50)